This is great, GB. It looks though that what they are measuring is the result of "LNP transfection with whatever DNA is floating around in the flask" Vs "LNP transfection with specified RNA and whatever is floating around in the flask".
Hence why both are producing the cGAS-STING (mapk) response with the cytokines that go with those pathways
Yup. Just think about network logic: The PI(4,5P)2 is a master regulator and electrostatic switch for at least 7 specific processes. You really need to read:
It’s a groundbreaking work showing you how the sequestration of PI4,5P2 and conversion into IP3, DAG, and PI(3,4,5)P3 is a key regulator for the whole signaling cascade network.
This is great, GB. It looks though that what they are measuring is the result of "LNP transfection with whatever DNA is floating around in the flask" Vs "LNP transfection with specified RNA and whatever is floating around in the flask".
Hence why both are producing the cGAS-STING (mapk) response with the cytokines that go with those pathways
Yup. Just think about network logic: The PI(4,5P)2 is a master regulator and electrostatic switch for at least 7 specific processes. You really need to read:
https://pubmed.ncbi.nlm.nih.gov/16319880/
It’s a groundbreaking work showing you how the sequestration of PI4,5P2 and conversion into IP3, DAG, and PI(3,4,5)P3 is a key regulator for the whole signaling cascade network.
1. LNPs are bioactive
2. LNPs are bioactive even without mRNA
3. clinical formulations have about a third of the total particles as empty or minimally loaded LNPs
No one is talking about this.
What is wrong with this picture?
Don’t ask me. I’m a chicken. Narf!